拟南芥DRE/CRT结合转录因子DREB2C的过表达增强了耐热性。
Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance.
作者信息
Lim Chan Ju, Hwang Jung Eun, Chen Huan, Hong Joon Ki, Yang Kyung Ae, Choi Man Soo, Lee Kyun Oh, Chung Woo Sik, Lee Sang Yeol, Lim Chae Oh
机构信息
Division of Applied Life Science (BK21), Environmental Biotechnology National Core Research Center and PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
出版信息
Biochem Biophys Res Commun. 2007 Oct 19;362(2):431-6. doi: 10.1016/j.bbrc.2007.08.007. Epub 2007 Aug 10.
The dehydration responsive element binding protein 2 (DREB2) subgroup belongs to the plant-specific APETALA2/ethylene-responsive element binding factor (AP2/ERF) family of transcription factors. We have characterized cDNA encoding Arabidopsis thaliana DREB2C, which is induced by mild heat stress. Both an electrophoretic mobility shift assay (EMSA) and a yeast one-hybrid assay revealed that DREB2C(145-528) was able to form a complex with the dehydration responsive element/C-repeat (DRE/CRT; A/GCCGAC) motif. A trans-activating ability test in yeast demonstrated that DREB2C could effectively function as a trans-activator. Constitutive expression of DREB2C under the control of the cauliflower mosaic virus (CaMV) 35S promoter led to enhanced thermotolerance in transgenic lines of Arabidopsis. Microarray and RT-PCR analyses of transgenic plants revealed that DREB2C regulates expression of several heat stress-inducible genes that contain DRE/CRT elements in their promoters. From these data, we deduced that DREB2C is a regulator of heat stress tolerance in Arabidopsis.
脱水响应元件结合蛋白2（DREB2）亚组属于植物特有的APETALA2/乙烯响应元件结合因子（AP2/ERF）转录因子家族。我们已对编码拟南芥DREB2C的cDNA进行了特征分析，该基因受轻度热胁迫诱导。电泳迁移率变动分析（EMSA）和酵母单杂交分析均表明，DREB2C（145-528）能够与脱水响应元件/C-重复序列（DRE/CRT；A/GCCGAC）基序形成复合物。酵母中的反式激活能力测试表明，DREB2C可有效作为反式激活因子发挥作用。在花椰菜花叶病毒（CaMV）35S启动子控制下的DREB2C组成型表达导致拟南芥转基因株系耐热性增强。对转基因植物的微阵列和RT-PCR分析表明，DREB2C调节其启动子中含有DRE/CRT元件的几个热胁迫诱导基因的表达。根据这些数据，我们推断DREB2C是拟南芥热胁迫耐受性的调节因子。
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