三种用于粪便去污以分离结核分枝杆菌的方法比较。

Comparison of three methods for decontamination of faeces for isolation of Mycobacterium tuberculosis.

作者信息

Allen B W

机构信息

Bacteriology Department, Royal Postgraduate Medical School, London, UK.

出版信息

Tubercle. 1991 Sep;72(3):214-7. doi: 10.1016/0041-3879(91)90011-g.

DOI:10.1016/0041-3879(91)90011-g

PMID:1771682

Abstract

Sputum and faeces were obtained from 276 patients on admission to a study of drug resistance in Hong Kong. Acid-fast bacilli were detected microscopically in 103 (37%) sputum specimens and 135 (49%) yielded Mycobacterium tuberculosis on culture. Three methods were used to decontaminate faeces prior to dilution and culture in selective liquid Kirchner medium. A total of 61 faecal specimens were positive for M. tuberculosis on culture and, of these, pretreatment with sodium hydroxide yielded 60 (98%), Portaels modification of Wolinsky and Rynearsons's method 28 (46%) and the combined use of benzalkonium chloride and 1-hexdecylpyridinium chloride yielded 32 (52%). It is recommended that faeces should be treated with sodium hydroxide followed by dilution and culture in selective media, although it may be necessary to formulate new selective media for mycobacterial species other than M. tuberculosis.

摘要

从276名入院患者处获取痰液和粪便，用于香港一项耐药性研究。在103份（37%）痰液标本中通过显微镜检测到抗酸杆菌，135份（49%）培养出结核分枝杆菌。在选择性液体基尔希纳培养基中稀释和培养之前，使用了三种方法对粪便进行去污处理。共有61份粪便标本培养出结核分枝杆菌，其中，用氢氧化钠预处理的有60份（98%），采用沃林斯基和赖尼尔森方法的波塔埃尔斯改良法有28份（46%），联合使用苯扎氯铵和十六烷基吡啶氯化物有32份（52%）。建议先用氢氧化钠处理粪便，然后在选择性培养基中进行稀释和培养，不过可能有必要为除结核分枝杆菌以外的分枝杆菌菌种配制新的选择性培养基。