Yi Yu-Chiao, Chen Ming-Jer, Ho Jason Yen-Ping, Guu Hwa-Fen, Ho Esther Shih-Chu
Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, 160, Chung-Kang Road Section 3, Taichung 407, Taiwan.
J Assist Reprod Genet. 2007 Oct;24(10):445-9. doi: 10.1007/s10815-007-9161-6. Epub 2007 Aug 29.
To evaluate the effect of mitochondrial transfer on embryonic development.
Mitochondria concentrates were collected from murine hepatocytes and fertilized murine zygotes from young and older mice in the 2PN stage were subjected to mitochondrial transfer and cultured in vitro to evaluate the embryonic development.
After extended in vitro culture, 37.65% and 20.91% embryos from the young mice developed to the blastocyst stage in the injected and control groups respectively, which is statistically significant. There was no difference in terms of hatching rates (1.76% and 1.82% respectively). Zygotes from the older mice (>20 weeks old) that received mitochondrial transfer also had a better developmental outcome than the control group (54.35% and 18.92% developed to morula stage, 43.48% and 8.11% developed to the blastocyst stage respectively), which is statistically significant.
Our results for the murine model provide direct scientific evidence that mitochondrial transfer improves embryonic development. However, potential risks such as mitochondrial heteroplasmy, nuclear-mitochondrial interaction and epigenetic aspects all deserve further evaluation before mitochondrial transfer is applied clinically.
评估线粒体转移对胚胎发育的影响。
从小鼠肝细胞中收集线粒体浓缩物，对处于2PN期的年轻和老年小鼠的受精小鼠受精卵进行线粒体转移，并在体外培养以评估胚胎发育。
经过延长的体外培养后，年轻小鼠注射组和对照组分别有37.65%和20.91%的胚胎发育到囊胚阶段，差异具有统计学意义。孵化率方面无差异（分别为1.76%和1.82%）。接受线粒体转移的老年小鼠（>20周龄）的受精卵也比对照组有更好的发育结果（分别有54.35%和18.92%发育到桑椹胚阶段，43.48%和8.11%发育到囊胚阶段），差异具有统计学意义。
我们对小鼠模型的研究结果提供了直接的科学证据，证明线粒体转移可改善胚胎发育。然而，在线粒体转移临床应用之前，线粒体异质性、核-线粒体相互作用和表观遗传学等潜在风险都值得进一步评估。
