Milici Maria Eleonora, Maida Carmelo Massimo, Spreghini Elisabetta, Ravazzolo Barbara, Oliveri Salvatore, Scalise Giorgio, Barchiesi Francesco
Dipartimento di Igiene e Microbioogia D'Alessandro, Università degli Studi di Palermo, Palermo, Italy.
J Clin Microbiol. 2007 Nov;45(11):3529-33. doi: 10.1128/JCM.00826-07. Epub 2007 Aug 29.
We compared the caspofungin (CAS) susceptibility testing results generated by the disk diffusion (DD) assay with the results of the Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BD) reference method for 106 yeast isolates. The isolates represented 11 different fungal species, including Candida albicans (n = 50), C. parapsilosis (n = 10), C. glabrata (n = 10), C. tropicalis (n = 10), C. guillermondii (n = 6), C. rugosa (n = 5), C. krusei (n = 5), C. kefyr (n = 2), C. pelliculosa (n = 2), Saccharomyces cerevisiae (n = 3), and Geotrichum candidum (n = 3). The DD assay was performed in supplemented Mueller-Hinton agar with CAS, which was tested at concentrations of 2, 10, and 25 mug per disk. MICs and inhibition zone diameters were evaluated at 24 and 48 h. In general, the results obtained by the DD assay correlated well with those obtained by the BD method. In particular, a significant correlation between methods was observed when CAS was used at concentration of 2 mug/disk at a reading time of either 24 or 48 h.
我们将纸片扩散法（DD）检测的卡泊芬净（CAS）药敏试验结果与临床和实验室标准协会（CLSI）肉汤微量稀释法（BD）参考方法对106株酵母分离株的检测结果进行了比较。这些分离株代表11种不同的真菌物种，包括白色念珠菌（n = 50）、近平滑念珠菌（n = 10）、光滑念珠菌（n = 10）、热带念珠菌（n = 10）、季也蒙念珠菌（n = 6）、皱落念珠菌（n = 5）、克柔念珠菌（n = 5）、凯菲念珠菌（n = 2）、膜璞念珠菌（n = 2）、酿酒酵母（n = 3）和白地霉（n = 3）。DD试验在添加了CAS的Mueller-Hinton琼脂中进行，每片纸片的CAS测试浓度为2、10和25μg。在24小时和48小时时评估最低抑菌浓度（MIC）和抑菌圈直径。总体而言，DD试验获得的结果与BD方法获得的结果相关性良好。特别是，当在24小时或48小时的读数时间使用2μg/片浓度的CAS时，观察到两种方法之间存在显著相关性。
