Science. 1937 Feb 12;85(2198):181-3. doi: 10.1126/science.85.2198.181.
A high molecular weight crystalline protein, possessing the properties of ring spot virus and differing markedly from tobacco mosaic virus protein in its physical, chemical and serological properties, has been isolated by means of an ultracentrifuge from Turkish tobacco plants diseased with tobacco ring spot virus. Ultracentrifugal methods were also used to demonstrate that high molecular weight proteins are characteristic of other virus diseases. The concentration of the different virus proteins in the host was found to differ greatly. The quantity ultracentrifuge, used in conjunction with an analytical ultracentrifuge, has proven to be a powerful tool for the concentration, purification and crystallization of high molecular weight virus proteins and to be indispensable in the case of unstable viruses existing in low concentration in the host.
已从感染烟草环斑病毒的土耳其烟草植株中,通过超速离心法分离到一种高分子量结晶蛋白。该蛋白具有环斑病毒的性质,且在物理、化学和血清学性质上与烟草花叶病毒蛋白有显著差异。超速离心法还表明,高分子量蛋白是其他病毒病的特征。还发现不同病毒蛋白在宿主中的浓度有很大差异。超速离心法与分析超速离心法相结合,已被证明是浓缩、纯化和结晶高分子量病毒蛋白的有力工具,对于在宿主中浓度低且不稳定的病毒,该方法是不可或缺的。