由于dcd基因缺失导致大肠杆菌K-12 P2诱导物中脱氧核糖核苷酸库的改变。
Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene.
作者信息
Neuhard J, Thomassen E
出版信息
J Bacteriol. 1976 May;126(2):999-1001. doi: 10.1128/jb.126.2.999-1001.1976.
Abstract
Deletion of the Escherichia coli K-12 chromosome associated with P2 mediated education extend through the structural gene for uridine kinase, udk, and the dcd gene encoding 2'-deoxycytidine 5'-triphosphate deaminase. The lack of uridine kinase makes a positive selection possible for these strains. Due to the dcd mutation, P2 eductants show large alterations in their deoxyribonucleoside triphosphate pools.
摘要
与P2介导的转导相关的大肠杆菌K-12染色体缺失延伸至尿苷激酶的结构基因udk以及编码2'-脱氧胞苷5'-三磷酸脱氨酶的dcd基因。尿苷激酶的缺失使得对这些菌株进行阳性选择成为可能。由于dcd突变，P2转导体在其三磷酸脱氧核糖核苷库中表现出巨大变化。
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