Weihe E, Nohr D, Michel S, Müller S, Zentel H J, Fink T, Krekel J
Department of Anatomy, Johannes Gutenberg-University, Mainz, Germany.
Int J Neurosci. 1991 Jul;59(1-3):1-23. doi: 10.3109/00207459108985446.
Light microscopic immunohistochemistry was employed to elucidate and compare the presence, distribution, and coexistence of various peptides, neuroendocrine markers and enzymes of the catecholamine pathway in nerves supplying lymphoid tissues in a variety of mammalian species. All lymphoid organs and tissues receive innervation by fibers containing dopamine-beta-hydroxylase and/or tyrosine hydroxylase, neural markers like protein gene product 9.5, synaptophysin and neurofilament and a varied spectrum of peptides. The prominent peptides were tachykinins (substance P, neurokinin A), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), and vasoactive intestinal polypeptide/peptide histidine isoleucine (VIP/PHI). Opioid innervation was variable. Double immunofluorescence revealed coexistence of tachykinins and CGRP and of tyrosine hydroxylase and NPY. A minor proportion of fibers showed coexistence of NPY and tachykinins and of VIP/PHI and tachykinins. The possible importance of the complex peptidergic innervation of lymphoid tissues in inflammation, allergy, inflammatory pain and psycho-neuro-immuno-endocrine network function is discussed. A special immunomodulatory role of the sensory neurons is suggested.
采用光镜免疫组织化学方法，以阐明和比较多种哺乳动物物种中，供应淋巴组织的神经中各种肽、神经内分泌标志物以及儿茶酚胺途径的酶的存在、分布和共存情况。所有淋巴器官和组织均接受含有多巴胺-β-羟化酶和/或酪氨酸羟化酶的纤维、诸如蛋白基因产物9.5、突触素和神经丝等神经标志物以及多种肽的神经支配。主要的肽包括速激肽（P物质、神经激肽A）、降钙素基因相关肽（CGRP）、神经肽Y（NPY）以及血管活性肠多肽/肽组氨酸异亮氨酸（VIP/PHI）。阿片样物质神经支配情况各异。双重免疫荧光显示速激肽与CGRP共存，以及酪氨酸羟化酶与NPY共存。一小部分纤维显示NPY与速激肽共存，以及VIP/PHI与速激肽共存。文中讨论了淋巴组织复杂的肽能神经支配在炎症、过敏、炎性疼痛以及神经-心理-免疫-内分泌网络功能中的潜在重要性。提示感觉神经元具有特殊的免疫调节作用。
