Callister S M, Schell R F, Lovrich S D
Microbiology Research Laboratory, Gundersen Medical Foundation, La Crosse, Wisconsin 54601.
J Clin Microbiol. 1991 Sep;29(9):1773-6. doi: 10.1128/jcm.29.9.1773-1776.1991.
We developed an in vitro assay showing that Borrelia burgdorferi organisms were killed by serum from patients with Lyme disease. Twenty of 20 Lyme disease serum samples caused B. burgdorferi killing in a range of 36 to 99% compared with the mean number of viable spirochetes when sera from 10 healthy individuals were used. The percentage of killing of B. burgdorferi increased with convalescent serum from patients with early Lyme disease. The borreliacidal activity was detectable in some sera diluted 640-fold and was abrogated after treatment with anti-human immunoglobulin G. In contrast, pooled or individual normal human serum did not cause a decrease in the number of viable B. burgdorferi. Borreliacidal activity was also not detected in sera from patients with relapsing fever, rocky mountain spotted fever, syphilis, mononucleosis, rheumatoid factor, or DNA antibodies. Our results show that borreliacidal activity can be used as a specific serodiagnostic test for detecting Lyme disease.
我们开发了一种体外检测方法，结果显示莱姆病患者血清可杀死伯氏疏螺旋体。与使用10名健康个体血清时的活螺旋体平均数相比，20份莱姆病血清样本中有20份导致伯氏疏螺旋体的杀伤率在36%至99%之间。早期莱姆病患者恢复期血清对伯氏疏螺旋体的杀伤百分比增加。在一些稀释640倍的血清中可检测到杀螺旋体活性，且在用抗人免疫球蛋白G处理后活性消失。相比之下，混合或个体正常人血清不会导致活的伯氏疏螺旋体数量减少。在回归热、落基山斑疹热、梅毒、单核细胞增多症、类风湿因子或DNA抗体患者的血清中也未检测到杀螺旋体活性。我们的结果表明，杀螺旋体活性可作为检测莱姆病的一种特异性血清诊断试验。
