Shurtz-Swirski R, Simon R J, Cohen Y, Barnea E R
Feto-Placental Endocrine Unit, Rappaport Institute, Haifa, Israel.
Placenta. 1991 Sep-Oct;12(5):521-31. doi: 10.1016/0143-4004(91)90028-e.
We investigated the effect of embryonal neural and adrenal tissues (7-14 weeks gestational age) upon beta hCG secretion by homologous placental explants in static and dynamic cultures. In static co-culture significant inhibition by SC and brain was noted at 7-9 weeks. Similarly, in superfusion, using a novel co-chambering technique there was a significant reduction in the area under the curve but not peak frequency of spontaneous pulsatile beta hCG secretion. Incubations with neural tissues 11 weeks and above caused a stimulatory effect upon beta hCG secretion in both models. The effect of adrenal tissue in static cultures was different, namely slightly inhibitory at 7-9 weeks and inhibitory at 11 weeks and above. In superfusion, the effect of adrenal tissue was not significant. Extracted neural tissue 7-9 weeks incubated with placental explants exhibited inhibitory effects upon beta hCG secretion as well. Buffer-based extracts of neural tissues effect was more pronounced than alcohol-based extracts regarding beta hCG secretion. The effect of extracts was dose-dependent and effects were noted up until a 2000-fold dilution. In contrast, the buffer SC extract had no effect on progesterone (P) secretion while the alcohol extract effect was inhibitory at 7-9 weeks and stimulatory at greater than 11 weeks. Superfused explants pattern of beta hCG secretion was inhibited by one minute pulse of the SC buffer extract. In conclusion, the human neural tissue of embryonal origin may modulate placental hCG and P secretion during early pregnancy.
我们研究了胚胎神经组织和肾上腺组织（妊娠7 - 14周龄）对同源胎盘外植体在静态和动态培养中β - hCG分泌的影响。在静态共培养中，7 - 9周时可见SC和脑对其有显著抑制作用。同样，在灌注实验中，使用一种新型的共室技术，自发搏动性β - hCG分泌曲线下面积显著减少，但峰值频率未受影响。11周及以上的神经组织孵育在两种模型中均对β - hCG分泌产生刺激作用。肾上腺组织在静态培养中的作用不同，即7 - 9周时略有抑制，11周及以上时抑制作用明显。在灌注实验中，肾上腺组织的作用不显著。7 - 9周的提取神经组织与胎盘外植体共同孵育时，对β - hCG分泌也表现出抑制作用。就β - hCG分泌而言，基于缓冲液的神经组织提取物的作用比基于酒精的提取物更显著。提取物的作用呈剂量依赖性，直至2000倍稀释仍有作用。相比之下，缓冲液SC提取物对孕酮（P）分泌无影响，而酒精提取物在7 - 9周时具有抑制作用，在大于11周时具有刺激作用。SC缓冲液提取物的1分钟脉冲可抑制灌注外植体的β - hCG分泌模式。总之，胚胎来源的人类神经组织可能在妊娠早期调节胎盘hCG和P的分泌。
