低葡萄糖浓度对椎间盘细胞耗氧率的影响。
Effects of low glucose concentrations on oxygen consumption rates of intervertebral disc cells.
作者信息
Huang Chun-Yuh C, Yuan Tai-Yi, Jackson Alicia R, Hazbun Larry, Fraker Christopher, Gu Wei Yong
机构信息
College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
出版信息
Spine (Phila Pa 1976). 2007 Sep 1;32(19):2063-9. doi: 10.1097/BRS.0b013e318145a521.
STUDY DESIGN
Investigation of the effects of low glucose concentrations on the oxygen consumption rates of intervertebral disc cells.
OBJECTIVES
To determine the oxygen consumption rate of porcine anulus fibrosus (AF) cells at different glucose concentrations and to examine the differences in the oxygen consumption rate between AF and nucleus pulposus (NP) cells at different glucose levels.
SUMMARY OF BACKGROUND DATA
Poor nutrient supply has been suggested as a potential mechanism for degeneration of the intervertebral disc (IVD). Distribution of nutrients in the IVD is strongly dependent on transport properties of the tissue and cellular metabolic rates. Previous studies have shown dependence of oxygen consumption rate of IVD cells on oxygen tension, pH levels, and glucose levels outside the physiologic range. However, the oxygen consumption rate of AF cells at in vivo glucose levels has not been investigated.
METHODS
IVD cells were isolated from the outer AF and NP of 4- to 5-month-old porcine lumbar discs. The changes in oxygen tension were recorded when cells were cultured in sealed metabolism chamber. The oxygen consumption rate of cells was determined by theoretical curve fitting using the Michaelis-Menten equation.
RESULTS
The outer AF cells cultured in high glucose medium (25 mmol/L) exhibited the lowest oxygen consumption rate, whereas no significant differences in oxygen consumption rates were found among outer AF cells cultured at physiologic glucose levels (i.e., 1 mmol/L, 2.5 mmol/L, 5 mmol/L). The oxygen consumption rate of NP cells was significantly greater than that of outer AF cells.
CONCLUSION
Since the oxygen consumption rates determined in this study are comparable to the findings in the literature, this study has developed a new alternative method for determining oxygen consumption rate. The oxygen consumption rates of IVD cells reported in this study will be valuable for theoretically predicting local oxygen concentrations in IVD, which can provide a better understanding of transport of oxygen in the discs.
研究设计
调查低葡萄糖浓度对椎间盘细胞耗氧率的影响。
目的
确定不同葡萄糖浓度下猪纤维环（AF）细胞的耗氧率，并检测不同葡萄糖水平时AF细胞与髓核（NP）细胞耗氧率的差异。
背景资料总结
营养供应不足被认为是椎间盘退变的一个潜在机制。椎间盘内营养物质的分布很大程度上取决于组织的转运特性和细胞代谢率。先前的研究表明，椎间盘细胞的耗氧率依赖于生理范围外的氧张力、pH值和葡萄糖水平。然而，体内葡萄糖水平下AF细胞的耗氧率尚未得到研究。
方法
从4至5月龄猪腰椎间盘的外层AF和NP中分离出椎间盘细胞。将细胞置于密封的代谢室中培养时记录氧张力的变化。使用米氏方程通过理论曲线拟合确定细胞的耗氧率。
结果
在高葡萄糖培养基（25 mmol/L）中培养的外层AF细胞耗氧率最低，而在生理葡萄糖水平（即1 mmol/L、2.5 mmol/L、5 mmol/L）下培养的外层AF细胞之间耗氧率未发现显著差异。NP细胞的耗氧率显著高于外层AF细胞。
结论
由于本研究中测定的耗氧率与文献中的结果相当，因此本研究开发了一种测定耗氧率的新方法。本研究报告的椎间盘细胞耗氧率对于从理论上预测椎间盘内局部氧浓度具有重要价值，这有助于更好地理解椎间盘内的氧运输。
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