Si Wen-Hui
Suzhou Agriculture College, Suzhou 215008, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Jun;27(6):1181-4.
An analytical method for the determination of ribonucleic acid by spectrophotometry was established. At the maximum absorption wavelength for neutral red in B-R buffer solution, and under the best conditions, the decrease in the absorbance was linear with the amount of ribonucleic acid. The linearity range was 0.0-9.0 microg x mL(-1), the detection limit was 0.52 microg x mL(-1), and the correlation coeffient was 0.999 8. This method was simple, rapid, and selective. So its application to the determination of ribonucleic acid was satisfactory. The reaction mechanism was that the electrostatic interaction leads to molecular association of RNA with neutral red, resulting in anti-ion permutation and bonding reaction.
建立了一种用分光光度法测定核糖核酸的分析方法。在B-R缓冲溶液中中性红的最大吸收波长处，在最佳条件下，吸光度的降低与核糖核酸的量呈线性关系。线性范围为0.0 - 9.0 μg·mL⁻¹，检测限为0.52 μg·mL⁻¹，相关系数为0.999 8。该方法简便、快速且具有选择性。因此，其用于核糖核酸的测定结果令人满意。反应机理是静电相互作用导致RNA与中性红发生分子缔合，从而产生反离子置换和键合反应。
