[中性红共振光散射法测定脱氧核糖核酸]

[Determination of deoxyribonucleic acid with neutral red by resonance light scattering method].

作者信息

Xiang H Y, Chen X M, Li S Q, Xia S, Liu A X

机构信息

Institute of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan 411105, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Dec;21(6):822-5.

PMID:12958905

Abstract

The method is based on the enhancement effect of the resonance light scattering (RLS) due to the long range assembly of neutral red (NR) on the molecular surface of DNA. The RLS of NR is greatly enhanced by DNA in the pH range of 5.0-7.0. The enhanced intensity of RLS at 335.0 nm is proportional to the concentration of DNA in the range 0-600 ng.mL-1. The detection limit is 12.8 ng.mL-1. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results.

摘要

该方法基于中性红（NR）在DNA分子表面的远程组装对共振光散射（RLS）的增强作用。在pH值为5.0 - 7.0的范围内，DNA能极大地增强NR的RLS。在335.0 nm处增强的RLS强度与0 - 600 ng·mL-1范围内的DNA浓度成正比。检测限为12.8 ng·mL-1。该方法简便、快速，已应用于混合样品中DNA的测定，结果令人满意。