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构建p16Slux，一种用于改进革兰氏阴性菌生物发光标记的新型载体。

Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria.

作者信息

Riedel Christian U, Casey Pat G, Mulcahy Heidi, O'Gara Fergal, Gahan Cormac G M, Hill Colin

机构信息

Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.

出版信息

Appl Environ Microbiol. 2007 Nov;73(21):7092-5. doi: 10.1128/AEM.01394-07. Epub 2007 Aug 31.

DOI:10.1128/AEM.01394-07
PMID:17766445
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2074938/
Abstract

A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.

摘要

已构建一种新型载体，用于通过位点特异性整合到细菌染色体的16S位点对革兰氏阴性菌进行组成型发光标记。使用该载体成功标记了多种革兰氏阴性病原体，并且该系统在活体动物的小鼠感染过程中得到了验证。

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