构建p16Slux，一种用于改进革兰氏阴性菌生物发光标记的新型载体。
Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria.
作者信息
Riedel Christian U, Casey Pat G, Mulcahy Heidi, O'Gara Fergal, Gahan Cormac G M, Hill Colin
机构信息
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
出版信息
Appl Environ Microbiol. 2007 Nov;73(21):7092-5. doi: 10.1128/AEM.01394-07. Epub 2007 Aug 31.
Abstract
A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.
摘要
已构建一种新型载体，用于通过位点特异性整合到细菌染色体的16S位点对革兰氏阴性菌进行组成型发光标记。使用该载体成功标记了多种革兰氏阴性病原体，并且该系统在活体动物的小鼠感染过程中得到了验证。
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