Kageyama Akiko, Takahashi Yoko, Ōmura Satoshi
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Int J Syst Evol Microbiol. 2007 Sep;57(Pt 9):2163-2166. doi: 10.1099/ijs.0.65069-0.
Strain KV-657(T) was isolated from a paddy field soil sample collected in Japan using GPM agar plates supplemented with catalase. The strain was a Gram-positive, aerobic organism that formed branching hyphae with ll-diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major menaquinone was MK-8(H(4)). Mycolic acids were not detected. The G+C content of the DNA was 70 mol%. 16S rRNA gene sequence analysis revealed that this strain is closely related to Intrasporangium calvum DSM 43043(T), with a similarity of 97.6 %. Based on the morphological, biochemical and chemotaxonomic properties of this strain and phylogenetic analysis, it was concluded that this isolate represents a new genus and species in the family Intrasporangiaceae, for which the name Humihabitans oryzae gen. nov., sp. nov. is proposed. The type strain of Humihabitans oryzae is KV-657(T) (=NRRL B-24470(T) =NBRC 101802(T)).
菌株KV-657(T)是从日本采集的稻田土壤样品中分离得到的,所用培养基为添加过氧化氢酶的GPM琼脂平板。该菌株为革兰氏阳性需氧菌,形成具分支的菌丝,以ll-二氨基庚二酸作为诊断性肽聚糖二氨基酸。主要甲基萘醌为MK-8(H(4))。未检测到分枝菌酸。DNA的G+C含量为70 mol%。16S rRNA基因序列分析表明,该菌株与空孢囊菌属的Calvum DSM 43043(T)密切相关,相似性为97.6%。基于该菌株的形态、生化和化学分类学特性以及系统发育分析,得出该分离物代表孢囊菌科中的一个新属和新种的结论,为此提出名为稻栖土生菌(Humihabitans oryzae)的新属名和新种名。稻栖土生菌的模式菌株为KV-657(T)(=NRRL B-24470(T)=NBRC 101802(T))。