Kageyama Akiko, Takahashi Yoko, Seki Tamae, Tomoda Hiroshi, Ōmura Satoshi
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2555-2559. doi: 10.1099/ijs.0.63799-0.
Three novel strains were isolated from a soil sample collected in Japan using GPM agar plates supplemented with superoxide dismutase and/or catalase. The strains were Gram-positive, catalase-positive, irregular rod-shaped bacteria with meso-diaminopimelic acid as a peptidoglycan diagnostic diamino acid, and the acyl type of the peptidoglycan was acetyl. The major menaquinone was MK-8(H4). Mycolic acids were not detected. The G+C content of the DNA was 72-73 mol%. On the basis of morphological and chemotaxonomic properties and a phylogenetic analysis using 16S rRNA gene sequences, these strains were classified as a novel genus and species, Oryzihumus leptocrescens gen. nov., sp. nov., in the family Intrasporangiaceae of the order Actinomycetales. The type strain is KV-628T (=NRRL B-24347T=JCM 12835T=NBRC 100762T).
使用添加了超氧化物歧化酶和/或过氧化氢酶的GPM琼脂平板，从日本采集的一份土壤样本中分离出三株新菌株。这些菌株为革兰氏阳性、过氧化氢酶阳性、不规则杆状细菌，以中-二氨基庚二酸作为肽聚糖诊断性二氨基酸，肽聚糖的酰基类型为乙酰基。主要的甲基萘醌是MK-8(H4)。未检测到分枝菌酸。DNA的G+C含量为72-73 mol%。根据形态学和化学分类学特性以及使用16S rRNA基因序列进行的系统发育分析，这些菌株被归类为一个新的属和种，即放线菌目内孢囊菌科的纤细稻栖菌属（Oryzihumus），新属，新种。模式菌株是KV-628T（=NRRL B-24347T=JCM 12835T=NBRC 100762T）。
