Lawrence W C
J Gen Virol. 1976 Apr;31(1):81-91. doi: 10.1099/0022-1317-31-1-81.
A method for the purification of enveloped infectious equine herpesvirus type 1 (EHV-1) is presented. Virus from cell culture fluids harvested at 48 h post infection was concentrated by sedimentation and partially purified by differential precipitation with ammonium sulphate. The final steps of purification consisted of two cycles of flotation of virus in pre-formed CsCl density gradients. Yields of infectious virus were about 30% (18 to 44%) of that present in starting material. As judged by electron microscopy, mixed radioisotope labelling, and absence of phosphohydrolase, virus preparations possessed a high degree of purity. Sedimentation of EHV-1 into CsCl density gradients resulted in low recovery of infectious virus. Flotation of virus in CsCl gradients, however, was not deleterious to infectivity of viral preparations.
本文介绍了一种纯化有包膜的感染性马疱疹病毒1型（EHV-1）的方法。感染后48小时收获的细胞培养液中的病毒通过沉降浓缩，并用硫酸铵进行分级沉淀进行部分纯化。纯化的最后步骤包括在预先形成的CsCl密度梯度中对病毒进行两轮浮选。感染性病毒的产量约为起始材料中病毒含量的30%（18%至44%）。通过电子显微镜、混合放射性同位素标记以及不存在磷酸水解酶判断，病毒制剂具有高度的纯度。EHV-1沉降到CsCl密度梯度中导致感染性病毒的回收率较低。然而，病毒在CsCl梯度中的浮选对病毒制剂的感染性没有损害。
