Yanagi K
J Virol. 1981 May;38(2):737-48. doi: 10.1128/JVI.38.2.737-748.1981.
The buoyant density characteristics of infectious particles of herpes simplex virus types 1 and 2 were studied by centrifugation in sucrose and cesium chloride density gradients with a high resolution and satisfactory infectivity recovery. It was shown that two populations of infectious virions differing in buoyant density coexisted, the difference being slight but definite. The ratio of heavy (H) to light (L) viral particles varied depending upon the solute used, the strains of virus, and the cell origin. Circumstances favoring degradation of viral infectivity tended to increase the H portion. Incubation at 37 degrees C largely converted L to H, and heating at 45 degrees C converted all virions to H without infectivity. The L to H conversion was irreversible, and no populations intermediate between L and H were clearly observed. Inactivation by UV light irradiation did not affect the density pattern. That H was not an artefact due to penetration of solutes, osmotic pressure, viral aggregation, or loss of the envelope was shown experimentally. A difference in the outer shape of particles between negatively stained L and H populations was demonstrated by electron microscopy. Both cell-released and cell-bound herpes simplex virus particles gave essentially the same result with respect to the above characteristics. The effect of limiting dilutions of antiserum was similar to that of mild thermal treatment, in that denser virions increased parallel to a decrease in less dense virions. Sensitization with early immunoglobulin G, composed mainly of complement-requiring neutralizing antibody, caused the density transition, and subsequent addition of complement resulted in a further increase in the buoyant density of the sensitized virions. The DNA in virus particles neutralized with immunoglobulin G plus complement remained resistant to DNase treatment. Possible implications of the phenomena are discussed.
通过在蔗糖和氯化铯密度梯度中进行离心，以高分辨率和令人满意的感染性回收率研究了1型和2型单纯疱疹病毒感染性颗粒的浮力密度特征。结果表明，存在两种浮力密度不同的感染性病毒粒子群体，差异虽小但很明确。重（H）病毒粒子与轻（L）病毒粒子的比例因所用溶质、病毒株和细胞来源而异。有利于病毒感染性降解的情况往往会增加H部分的比例。在37℃孵育很大程度上将L转化为H，而在45℃加热则将所有病毒粒子转化为无感染性的H。L向H的转化是不可逆的，未清楚观察到L和H之间的中间群体。紫外线照射灭活不影响密度模式。实验表明，H不是由于溶质渗透、渗透压、病毒聚集或包膜丢失造成的假象。电子显微镜显示了负染的L和H群体之间粒子外形的差异。细胞释放的和细胞结合的单纯疱疹病毒粒子在上述特征方面基本得到相同的结果。抗血清有限稀释的效果与温和热处理的效果相似，即密度较大的病毒粒子增加，同时密度较小的病毒粒子减少。用主要由需要补体的中和抗体组成的早期免疫球蛋白G致敏导致密度转变，随后添加补体导致致敏病毒粒子的浮力密度进一步增加。用免疫球蛋白G加补体中和的病毒粒子中的DNA仍对DNA酶处理有抗性。讨论了这些现象可能的意义。
