Végh Z, Kremmer T, Boldizsár M, Gesztesi K A, Szajáni B
Department of Biochemistry, National Institute of Oncology, Budapest, Hungary.
Clin Chim Acta. 1991 Dec 16;203(2-3):259-68. doi: 10.1016/0009-8981(91)90298-q.
The origin of serum sialic acid measured in the lipid-bound sialic acid determination reported by Katopodis et al. (1980) was investigated in detail. By varying the experimental conditions of sample preparation the protein, lipid and sialic acid contents of the methanol-water extract obtained from human sera were analyzed and compared in healthy controls and cancer patients. Using polyacrylamide gel electrophoretic and gel chromatographic methods it has been shown that most of the lipid-bound sialic acid was attributed to the acid alpha 1-glycoprotein (orosomucoid) fraction of human sera. Based on these observations a re-evaluation of the molecular background of the LBSA determination seems to be necessary.
详细研究了卡托波迪斯等人（1980年）报道的脂质结合唾液酸测定中所测血清唾液酸的来源。通过改变样品制备的实验条件，对从健康对照者和癌症患者的人血清中获得的甲醇 - 水提取物中的蛋白质、脂质和唾液酸含量进行了分析和比较。使用聚丙烯酰胺凝胶电泳和凝胶色谱方法已表明，大部分脂质结合唾液酸归因于人血清的酸性α1 - 糖蛋白（类粘蛋白）部分。基于这些观察结果，似乎有必要对脂质结合唾液酸测定的分子背景进行重新评估。
