Korochkin L, Saveliev S, Ivanov A, Evgeniev M, Bessova N, Gulimova V
Institute of Developmental Biology, Moscow, USSR.
Genetica. 1991;85(1):23-34. doi: 10.1007/BF00056103.
Fragments of the neural primordium of a new Notch mutant of Drosophila melanogaster produced in our laboratory were transplanted into the neural tube of embryos of 4 amphibian species (caudate and ecaudate) immediately after completion of neurulation. The grafts were identified by using a light microscope, scanning electron miscroscope, and in situ hybridization with mobile genetic elements of Drosophila and fluorescent dyes as markers. As has been shown, Drosophila nerve cells survive and differentiate inside the neural tube of amphibian embryos. The grafts increase in size by twentyfold and the cell proliferation zones are retained during the period of six months. Differentiated cells of the graft formed axon-dendritic contacts with recipient cells and penetrated into the organisms' brain structures. The effect of Drosophila transplants proved to be different for caudate and ecaudate amphibians. The presence of the graft accelerated the development of Xenopus laevis and it also affected their behavior. This approach can be very useful for the study of genetic basis of development and behavior.
在神经胚形成完成后，立即将我们实验室培育出的一种新的果蝇Notch突变体的神经原基片段移植到4种两栖动物（有尾目和无尾目）胚胎的神经管中。通过光学显微镜、扫描电子显微镜以及使用果蝇的移动遗传元件和荧光染料作为标记进行原位杂交来识别移植体。结果表明，果蝇神经细胞在两栖动物胚胎的神经管内能够存活并分化。移植体的大小增加了20倍，并且在六个月的时间内细胞增殖区域得以保留。移植体的分化细胞与受体细胞形成了轴突 - 树突连接，并侵入到生物体的脑结构中。果蝇移植体对有尾目和无尾目两栖动物的影响有所不同。移植体的存在加速了非洲爪蟾的发育，并且还影响了它们的行为。这种方法对于研究发育和行为的遗传基础可能非常有用。
