McMillan J H, Cox G G, Kimler B F, Spicer J S, Batnitzky S
Department of Diagnostic Radiology, University of Kansas Medical Center, Kansas City 66103.
Magn Reson Imaging. 1991;9(4):553-8. doi: 10.1016/0730-725x(91)90042-k.
We have used an intracranial 9L rat brain tumor model to determine whether a novel metalloporphyrin, Mn[III] uroporphyrin I (MnUROP-I), could function as an intravenous MRI contrast agent for brain tumors. In several experiments, 24 male Fischer 344 rats were inoculated intracranially with 9L brain tumor cells. On day 15 postinoculation, animals were anesthetized and the femoral vein exposed. Prior to the intravenous injection of the contrast agent, a precontrast scan (1 Tesla in a standard head coil) was performed. Thirty min after injection of the contrast agent, a postcontrast scan was performed. Although there was only a suggestion of abnormality on the precontrast scans, the presence of tumor was visibility enhanced in the postcontrast scans. In 3 animals scanned at 24 hr postinjection, persistent tumor enhancement was demonstrated. Measured tumor sizes on the MRI scans were consistent with sizes measured at autopsy and histologically. These results demonstrate that MnUROP-I is an effective MRI contrast agent for the detection of an intracranial brain tumor in the rat model.
我们使用了一种颅内9L大鼠脑肿瘤模型来确定一种新型金属卟啉——锰（III）尿卟啉I（MnUROP-I）是否可以作为脑肿瘤的静脉注射磁共振成像（MRI）造影剂。在几个实验中，24只雄性Fischer 344大鼠被颅内接种9L脑肿瘤细胞。接种后第15天，动物被麻醉，股静脉暴露。在静脉注射造影剂之前，进行了一次造影前扫描（在标准头部线圈中使用1特斯拉）。注射造影剂30分钟后，进行了造影后扫描。尽管在造影前扫描中仅提示有异常，但在造影后扫描中肿瘤的存在变得更明显可见。在注射后24小时扫描的3只动物中，显示出持续的肿瘤强化。MRI扫描测量的肿瘤大小与尸检和组织学测量的大小一致。这些结果表明，MnUROP-I是大鼠模型中检测颅内脑肿瘤的一种有效MRI造影剂。
