Yamamoto T, Matsumura A, Shibata Y, Fujimori H, Nakai K, Yoshida F, Nose T, Sakata I, Nakajima S, Miwa N
Department of Neurosurgery, Institute of Clinical Medicine, University of Tsukuba, Tsukuba City, Ibaraki, Japan.
Neurosurgery. 1998 Jun;42(6):1332-7; discussion 1337-8. doi: 10.1097/00006123-199806000-00083.
We examined whether selective tumor accumulation of a novel manganese-metalloporphyrin (ATN-10) occurs in Fisher rats bearing intracerebral 9L gliomas.
After intravenous administration of ATN-10, magnetic resonance imaging of brains with tumors or nontumoral vasogenic brain edema was performed. Tissue manganese concentrations were measured by inductively coupled plasma atomic emission spectroscopy until 48 hours after administration of ATN-10, to evaluate its uptake in tumor, normal brain, and peritumoral brain tissue.
In magnetic resonance imaging scans, early enhancement was observed in both tumor tissue and regions of nontumoral vasogenic brain edema at 5 minutes after ATN-10 administration. However, delayed enhancement was noted only in tumor tissue, at 24 hours after intravenous injection of ATN-10. Comparison of rat brain specimens and 24-hour magnetic resonance imaging scans revealed that only the viable portions of tumors were enhanced with ATN-10; necrotic regions and areas of peritumoral brain tissue and nontumoral vasogenic edema were not. Significantly greater uptake of ATN-10 was found in tumor samples, compared with normal and peritumoral brain tissue, at 24 hours. A high tumor/normal brain tissue ratio (10.4) was achieved at 24 hours.
ATN-10, a manganese-metalloporphyrin, is a potentially useful tumor-localizing agent that accumulates and is preferentially retained in viable tumor tissue.
我们研究了新型锰金属卟啉（ATN-10）在患有脑内9L胶质瘤的Fisher大鼠中是否存在选择性肿瘤蓄积。
静脉注射ATN-10后，对患有肿瘤或非肿瘤性血管源性脑水肿的大鼠脑部进行磁共振成像。通过电感耦合等离子体原子发射光谱法测量组织锰浓度，直至注射ATN-10后48小时，以评估其在肿瘤、正常脑组织和肿瘤周围脑组织中的摄取情况。
在磁共振成像扫描中，注射ATN-10后5分钟，肿瘤组织和非肿瘤性血管源性脑水肿区域均出现早期强化。然而，静脉注射ATN-10后24小时，仅肿瘤组织出现延迟强化。对大鼠脑标本和24小时磁共振成像扫描的比较显示，只有肿瘤的存活部分被ATN-10强化；坏死区域、肿瘤周围脑组织区域和非肿瘤性血管源性水肿区域未被强化。在24小时时，肿瘤样本中ATN-10的摄取量明显高于正常脑组织和肿瘤周围脑组织。24小时时实现了较高的肿瘤/正常脑组织比率（10.4）。
锰金属卟啉ATN-10是一种潜在有用的肿瘤定位剂，它在存活的肿瘤组织中蓄积并优先保留。
