Masuzawa T, Okada Y, Beppu Y, Oku T, Kawamori F, Yanagihara Y
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Microbiol Immunol. 1991;35(10):913-9. doi: 10.1111/j.1348-0421.1991.tb02030.x.
Three strains of spirochetes (IKA1 to 3) were isolated from the midgut of Ixodes ovatus collected in the Ikawa region of the northern part of Shizuoka, Japan. These isolates had eight flagella, and their size and other morphological features were similar to Borrelia burgdorferi. They showed similar motility and reacted with monoclonal antibody (MAb) H9724 against borrelial flagella and with MAb H5332 against the outer surface protein A. These strains showed similar SDS-PAGE profiles to that of B. burgdorferi strain B31 and P/Bi isolated in the U.S.A. and Europe, respectively. Immunoblot with Lyme disease patient serum showed positive reactions with the flagella (41 Kilodalton, kDa), protein C (20 to 22 kDa), and outer surface protein A (29 kDa) of the isolates. Immunological properties, morphological characteristics, and epidemiological features revealed that these isolates were B. burgdorferi.
从日本静冈北部伊川地区采集的卵形硬蜱中肠分离出三株螺旋体（IKA1至3）。这些分离株有八条鞭毛，其大小和其他形态特征与伯氏疏螺旋体相似。它们表现出相似的运动性，与抗疏螺旋体鞭毛的单克隆抗体（MAb）H9724以及抗外表面蛋白A的单克隆抗体H5332发生反应。这些菌株的SDS-PAGE图谱分别与在美国和欧洲分离出的伯氏疏螺旋体菌株B31和P/Bi相似。用莱姆病患者血清进行免疫印迹显示，分离株的鞭毛（41千道尔顿，kDa）、蛋白C（20至22 kDa）和外表面蛋白A（29 kDa）呈阳性反应。免疫学特性、形态特征和流行病学特征表明，这些分离株为伯氏疏螺旋体。
