Masuzawa T, Okada Y, Yanagihara Y, Sato N
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
J Clin Microbiol. 1991 Aug;29(8):1568-73. doi: 10.1128/jcm.29.8.1568-1573.1991.
Spirochete strains HP3 and HO14, isolated from Ixodes persulcatus and I. ovatus in Hokkaido in 1989, were the first isolates of Borrelia burgdorferi, the etiological agent of Lyme disease, to be recognized in Japan. Antigenic properties of the Japanese strains were compared with those of the strains isolated in the United States (B31 and 297) and Europe (IRS, P/Gau, P/Bi, 2/B45, and 3/B56) by Western blotting (immunoblotting), by using monoclonal antibodies (MAbs) against strains B31 and P/Bi. The Japanese strains reacted with MAb U40 against the 41-kDa antigen. MAb E34a31 against Osp A reacted with all the strains tested except for strain HP3. Furthermore, MAb U31b against Osp A reacted with all the American and European strains but did not react with the Japanese strains. When MAbs against Osp B were used, MAb E34b reacted only with European strains and MAb U34b reacted only with the American strains. However, neither showed reactivity to two Japanese strains. MAb E60 against 60-kDa antigen reacted with all the U.S. and European strains and strain HP3 but did not react with Japanese strain HO14. These results indicate that the antigenicity of the Japanese strains isolated from two species of ixodid ticks is different from that of the strains isolated in the United States and Europe. It is suggested that the Japanese strains are much more suitable than the U.S. or European strains as the antigen source for the serodiagnosis of Lyme disease in Japan.
1989年从北海道的全沟硬蜱和卵形硬蜱中分离出的螺旋体菌株HP3和HO14，是日本首次确认的莱姆病病原体——伯氏疏螺旋体的分离株。通过蛋白质印迹法（免疫印迹法），使用针对菌株B31和P/Bi的单克隆抗体（MAb），将日本菌株的抗原特性与在美国（B31和297）和欧洲（IRS、P/Gau、P/Bi、2/B45和3/B56）分离的菌株进行了比较。日本菌株与针对41 kDa抗原的单克隆抗体U40发生反应。针对Osp A的单克隆抗体E34a31与除菌株HP3外的所有测试菌株发生反应。此外，针对Osp A的单克隆抗体U31b与所有美国和欧洲菌株发生反应，但与日本菌株不发生反应。当使用针对Osp B的单克隆抗体时，单克隆抗体E34b仅与欧洲菌株发生反应，单克隆抗体U34b仅与美国菌株发生反应。然而，两者均未显示出与两种日本菌株的反应性。针对60 kDa抗原的单克隆抗体E60与所有美国和欧洲菌株以及菌株HP3发生反应，但与日本菌株HO14不发生反应。这些结果表明，从两种硬蜱中分离出的日本菌株的抗原性与在美国和欧洲分离出的菌株不同。有人认为，日本菌株比美国或欧洲菌株更适合作为日本莱姆病血清学诊断的抗原来源。
