[清风痛胶囊的高效液相色谱指纹图谱测定]

[Determination of HPLC fingerprint of Qingfengtong capsule].

作者信息

Zhang Yong-Xin, Yang Hua, Zhang Qi-Wei, Yi Hong, Chen Gui-Pin

机构信息

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(23):2492-3.

PMID:18330240

Abstract

OBJECTIVE

To establish a fingerprint analysis method of Qingfengtong capsule by HPLC.

METHOD

The samples were extracted with 70% ethanol in an ultrasonic bath. The extracts were analyzed at 35 degrees C on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) with 0.1 mol x L(-1) potassium dihydrogen phosphate water-solution as mobile phase A, and methanol as mobile phase B. The analysis followed a linear gradient elution program. Initial condition of the mobile phase was 10% B for 2 minutes; then changed to 90% B in 40 minutes. The flow rate was kept at 1.0 ml x min(-1) and the detector wavelength was 262 nm.

RESULT

The main peaks in fingerprint chromatogram of Qingfengtong capsules were separated fairly well. The results of method validation meet the requirements for the fingerprints.

CONCLUSION

The established method can be used for the quality control of Qingfengtong capsules.

摘要

目的

建立清风痛胶囊的高效液相色谱指纹图谱分析方法。

方法

样品用70%乙醇在超声浴中提取。提取物在35℃下，于Diamonsil C18柱（4.6 mm×250 mm，5μm）上进行分析，以0.1 mol·L⁻¹磷酸二氢钾水溶液为流动相A，甲醇为流动相B。分析采用线性梯度洗脱程序。流动相初始条件为10%B，保持2分钟；然后在40分钟内变为90%B。流速保持在1.0 ml·min⁻¹，检测波长为262 nm。