[高效液相色谱法同时测定青海和四川獐牙菜中六种主要成分]
[Simultaneous determination of six main constituents in Swertia of Qinghai Province and Sichuan Province by HPLC].
作者信息
Bao Yi, Ji Wen-He, Ma Yu-Hua, Ji Lan-Ju
机构信息
Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Dec;31(24):2036-8.
OBJECTIVE
To establish a quantitative method of simultaneously determination of swertiamarin, gentiopicroside, mangiferin, swertianolin, isoorientin, 1,8-drihydroxy-3-methoxy-xthanone in Swertia from Qinghai province and Sichuan province by HPLC.
METHOD
The samples were separated on the column of Kromasil C18 (4. 6 mm x 250 mm, 5 microm) which eluted with methanol and water (content 0.02% phosphoric acid). The ratio of methanol increased from 20% to 80% during 20-50 min, and from 80% to 100% during 50-60 min, with detected wavelength 254 nm, flow rate at 1 mL x min(-1), column temperature 35 degrees C.
RESULT
Six compounds were base-isolated, the linear ranges of swertiamarin, gentiopicroside, mangiferin, 4-swertianolin, 5-isoorientin, 1,8-drihydroxy-3-methoxy-xthanone were excellent.
CONCLUSION
The method was rapid and precise, and can be use for controlling medicinal materials quality.
目的
建立高效液相色谱法同时测定青海和四川产獐牙菜中獐牙菜苦苷、龙胆苦苷、芒果苷、獐牙菜苷、异荭草苷、1,8 - 二羟基 - 3 - 甲氧基口山酮含量的定量方法。
方法
采用Kromasil C18柱（4.6 mm×250 mm，5 μm），以甲醇 - 水（含0.02%磷酸）为流动相进行梯度洗脱，甲醇比例在20 - 50 min内由20%升至80%，50 - 60 min内由80%升至100%，检测波长254 nm，流速1 mL·min⁻¹，柱温35℃。
结果
6种化合物得到基线分离，獐牙菜苦苷、龙胆苦苷、芒果苷、4 - 獐牙菜苷、5 - 异荭草苷、1,8 - 二羟基 - 3 - 甲氧基口山酮线性关系良好。
结论
该方法快速、准确，可用于獐牙菜药材质量控制。
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