Yang Yong, Luo Wei-Zao, Liu Xiang, Wang Chang-Hua, Zhao Ji-Feng, Qin Song-Yun, Zhong Guo-Yue
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Zhongguo Zhong Yao Za Zhi. 2012 Oct;37(20):3141-6.
To establish a method for determination of 10 ingredients such as gentiopicroside, sweroside, and mangiferin in India swertia, and settle the index components and their limits.
By Welch materials AQ-C18 column, determination was conducted by the gradient elution with methanol and 0.4% formic acid as mobile phase, with column temperature 30 degrees C, flow rate at 1.0 mL x min(-1), and 254 nm as the detection wavelength.
The linear relatives of 10 ingredients were good. The method showed the high precision and good reproducibility, and recovery rates were between 97% and 103%. The ingredients of market com-modities varied greatly.
This method is simple, sensitive, reproducible, and applicable to the determination of the main ingredients in India Swertia. Sweroside and mango glycosides were suggested as the index components for determination in Jia Di (Swertia chirayita), and their content limits are not less than 0.1%, 0.3%, respectively.
建立印度獐牙菜中龙胆苦苷、獐牙菜苷、芒果苷等10种成分的测定方法，确定其指标成分及限度。
采用Welch材料AQ-C18柱，以甲醇和0.4%甲酸为流动相进行梯度洗脱，柱温30℃，流速1.0 mL·min⁻¹，检测波长254 nm进行测定。
10种成分线性关系良好。该方法精密度高、重现性好，回收率在97%~103%之间。市售商品成分差异较大。
该方法简便、灵敏、重现性好，适用于印度獐牙菜中主要成分的测定。建议将獐牙菜苷和芒果苷作为加蒂（印度獐牙菜）的测定指标成分，其含量限度分别不得低于0.1%、0.3%。
