Wang Bin, Guan Si-ming, Li Xi-dong
Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1207-10.
To investigate the effects of the micrometer compound rhizoma coptidis on inflammatory factors and its possible mechanism in rabbit fed with high-lipid food.
The levels of CRP, IL-1 and TNF-alpha were all determinated by ELISA method. The mRNA and activity of NF-kappaB were determinated by RT-PCR and EMSA, respectively.
The level of CRP, IL-1 and TNF-alpha were significantly increased by feeding for 16 weeks with high-lipid diet in rabbit. It was significantly increased that the mRNA and the binging activity with DNA of NF-kappaB in thorax aorta of rabbits fed by high-lipid diet, too. The micrometer compound rhizoma coptidis can reverse the effects of high-lipid diet on CRP, IL-1, TNF-alpha and NF-kappaB.
The micrometer compound rhizoma coptidis can inhibit the expression of inflammatory factor possibly through inhibitting the expression and activity of NF-kappaB.
研究微米级复方黄连对高脂饮食家兔炎症因子的影响及其可能机制。
采用ELISA法检测CRP、IL-1和TNF-α水平。分别用RT-PCR和EMSA法检测NF-κB的mRNA和活性。
高脂饮食喂养家兔16周后，CRP、IL-1和TNF-α水平显著升高。高脂饮食喂养家兔胸主动脉中NF-κB的mRNA及与DNA的结合活性也显著升高。微米级复方黄连可逆转高脂饮食对CRP、IL-1、TNF-α和NF-κB的影响。
微米级复方黄连可能通过抑制NF-κB的表达和活性来抑制炎症因子的表达。
