使用合成肽库评估蛋白质法尼基转移酶底物特异性。
Evaluation of protein farnesyltransferase substrate specificity using synthetic peptide libraries.
作者信息
Krzysiak Amanda J, Scott Sarah A, Hicks Katherine A, Fierke Carol A, Gibbs Richard A
机构信息
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA.
出版信息
Bioorg Med Chem Lett. 2007 Oct 15;17(20):5548-51. doi: 10.1016/j.bmcl.2007.08.024. Epub 2007 Aug 16.
Abstract
Farnesylation, catalyzed by protein farnesyltransferase (FTase), is an important post-translational modification guiding cellular localization. Recently predictive models for identifying FTase substrates have been reported. Here we evaluate these models through screening of dansylated-GCaaS peptides, which also provides new insights into the protein substrate selectivity of FTase.
摘要
由蛋白质法尼基转移酶（FTase）催化的法尼基化是一种指导细胞定位的重要翻译后修饰。最近有报道称建立了用于识别FTase底物的预测模型。在此，我们通过筛选丹磺酰化的GCaaS肽来评估这些模型，这也为FTase的蛋白质底物选择性提供了新的见解。
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