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脱氧胆酸钠和Sephacryl-200在高密度脂蛋白脱脂及分离中的应用

The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.

作者信息

Robern H

出版信息

Experientia. 1982 Apr 15;38(4):437-9. doi: 10.1007/BF01952626.

DOI:10.1007/BF01952626
PMID:7084403
Abstract

A method to remove lipids from human plasma high-density lipoprotein (HDL) was developed. The procedure required column chromatography on Sephacryl-200 in the presence of the bile salt sodium deoxycholate. The lipid free protein obtained retained the immunological properties of the native HDL.

摘要

已开发出一种从人血浆高密度脂蛋白（HDL）中去除脂质的方法。该程序需要在脱氧胆酸钠盐存在的情况下，在Sephacryl-200上进行柱色谱法。所获得的无脂质蛋白质保留了天然HDL的免疫特性。

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The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.脱氧胆酸钠和Sephacryl-200在高密度脂蛋白脱脂及分离中的应用
Experientia. 1982 Apr 15;38(4):437-9. doi: 10.1007/BF01952626.
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本文引用的文献

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The behavior of glyceride-fatty acid mixtures in bile salt solution: Studies by gel filtration.甘油酯-脂肪酸混合物在胆盐溶液中的行为：凝胶过滤研究
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Phosphorus assay in column chromatography.柱色谱法中的磷测定
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Forms of human serum high density lipoprotein protein.人血清高密度脂蛋白蛋白质的形式。
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Enzymatic determination of total serum cholesterol.血清总胆固醇的酶法测定
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Characterization of plasma lipoproteins separated and purified by agarose-column chromatography.通过琼脂糖柱色谱法分离和纯化的血浆脂蛋白的表征
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