Keith A D, Gauslaa G, Anderson B S
Biology Department, University of Oregon, Eugene, Oregon.
Lipids. 1967 Sep;2(5):429-31. doi: 10.1007/BF02531859.
A new in vitro technique, utilizing ruptured larvae ofDrosophila melanogaster, was employed to study the incorporation of(3)H-acetate into long-chain fatty acids. Preparative gas-liquid chromatography and scintillation spectroscopy were used to determine the relative activity of each fatty acid from total lipid extracts. Quantitative changes were observed in the distribution of label during the course of the incubation times, which ranged from five minutes to nine hours. All fatty acids which were observed to incorporate acetate in previous in vivo studies also showed incorporation of label under these in vitro conditions. It is concluded that this system may be useful for studying aspects on insect metabolism for short intervals of time.
一种利用黑腹果蝇破裂幼虫的新体外技术，被用于研究（3）H-乙酸盐掺入长链脂肪酸的情况。采用制备气相色谱法和闪烁光谱法来测定总脂质提取物中每种脂肪酸的相对活性。在从5分钟到9小时的孵育过程中，观察到标记分布的定量变化。在之前的体内研究中观察到能掺入乙酸盐的所有脂肪酸，在这些体外条件下也显示出标记的掺入。结论是，该系统可能有助于在短时间内研究昆虫代谢的各个方面。
