Teague B D, Clark A G, Doane W W
J Exp Zool. 1986 Oct;240(1):95-104. doi: 10.1002/jez.1402400112.
A comparative developmental analysis was made of lipids from wild-type and adipose60 (adp60) mutants of Drosophila melanogaster. The lipid content and fatty acid profiles of late third instar larvae, pupae, and mature adults were characterized in methanol:chloroform extracts utilizing thin layer and gas-liquid chromatography. Total lipid content of mutant adults was approximately twice that of the wild-type, but no genotypic differences in lipid content were seen in earlier developmental stages. No sexual dimorphism was observed in total lipid content, although fatty acid profiles revealed some sexual differences. Many stage-specific differences in fatty acid profiles and lipid content were developmentally associated with each genotype. Mutants tended to retain the larval phenotype in lipid content and, to a lesser extent, in fatty acid profile. In comparison to wild-type, mutants tended to have increased lipid saturation, especially in 16-carbon fatty acids in mature adults and in 18:0 fatty acids in late larvae and pupae. No significant difference between the mutants and wild-type appeared in the developmental profiles for 14:1 fatty acid isomers. Hence, adp60 does not alter the desaturation-elongation pathway, a secondary pathway for fatty acid desaturation in Drosophila, which received support from this analysis.
对黑腹果蝇野生型和脂肪60（adp60）突变体的脂质进行了比较发育分析。利用薄层色谱和气相色谱法对甲醇：氯仿提取物中三龄晚期幼虫、蛹和成熟成虫的脂质含量和脂肪酸谱进行了表征。突变体成虫的总脂质含量约为野生型的两倍，但在早期发育阶段未观察到脂质含量的基因型差异。尽管脂肪酸谱显示出一些性别差异，但在总脂质含量上未观察到性别二态性。脂肪酸谱和脂质含量的许多阶段特异性差异在发育上与每种基因型相关。突变体在脂质含量上倾向于保留幼虫表型，在脂肪酸谱上保留程度较小。与野生型相比，突变体的脂质饱和度倾向于增加，尤其是在成熟成虫的16碳脂肪酸以及晚期幼虫和蛹的18:0脂肪酸中。突变体与野生型之间在14:1脂肪酸异构体的发育谱上没有显著差异。因此，adp60不会改变去饱和-延长途径，这是果蝇脂肪酸去饱和的次要途径，该分析支持了这一点。
