苏丹东部某村庄恶性疟原虫的遗传多样性。2. 近期分离株的耐药性、分子核型及mdr1基因型

Genetic diversity of Plasmodium falciparum in a village in eastern Sudan. 2. Drug resistance, molecular karyotypes and the mdr1 genotype of recent isolates.

作者信息

Babiker H A, Creasey A M, Bayoumi R A, Walliker D, Arnot D E

机构信息

Institute of Cell, Animal and Population Biology, University of Edinburgh, Scotland, UK.

出版信息

Trans R Soc Trop Med Hyg. 1991 Sep-Oct;85(5):578-83. doi: 10.1016/0035-9203(91)90349-4.

DOI:10.1016/0035-9203(91)90349-4

PMID:1780979

Abstract

Isolates of Plasmodium falciparum from a Sudanese village have been collected as part of a study of parasite genetic diversity during seasonal malaria epidemics. The sensitivity in vitro to chloroquine, pyrimethamine and mefloquine of these isolates has been determined. To assess the utility of pulse field gel chromosome separations in isolate characterization, 18 samples from individual patients in a single village were studied using this technique. Extensive variation in chromosome size was detected, no 2 isolates having identical molecular karyotypes. No multidrug resistance (mdr) gene amplification polymorphisms were detected in either chloroquine-resistant or chloroquine-sensitive isolates in this sample.

摘要

作为季节性疟疾流行期间寄生虫遗传多样性研究的一部分，已从苏丹一个村庄收集了恶性疟原虫分离株。已测定了这些分离株对氯喹、乙胺嘧啶和甲氟喹的体外敏感性。为了评估脉冲场凝胶染色体分离在分离株鉴定中的实用性，使用该技术研究了来自单个村庄的18例患者的样本。检测到染色体大小存在广泛差异，没有两个分离株具有相同的分子核型。在该样本中，耐氯喹或氯喹敏感的分离株均未检测到多药耐药（mdr）基因扩增多态性。