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Comparison of the adhesion properties of Deleya marina and the exopolysaccharide-defective mutant strain DMR.

作者信息

Shea C, Nunley J W, Williamson J C, Smith-Somerville H E

机构信息

Department of Biological Sciences, University of Alabama, Tuscaloosa 35487.

出版信息

Appl Environ Microbiol. 1991 Nov;57(11):3107-13. doi: 10.1128/aem.57.11.3107-3113.1991.

DOI:10.1128/aem.57.11.3107-3113.1991
PMID:1781675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC183934/
Abstract

Deleya marina 219 (ATCC 25374) produces large quantities of an acidic exopolysaccharide and characteristically forms mucoid colonies and large aggregates of cells. The exopolysaccharide of wild-type D. marina cells appears to occur as both film and fibrils in electron micrographs. The organization of exopolymeric material was indicative of structural heterogeneity. A spontaneous rough-colony mutant defective in exopolysaccharide, D. marina DMR, has been isolated. The absence of exopolymer corresponds to a nonmucoid, nonaggregating, adhesion-altered phenotype. In microplate adhesion assays, wild-type cells grown at 19 or 25 degrees C attached to hydrophilic surfaces but not to a hydrophobic surface. In contrast, mutant cells exhibited a significantly reduced level of attachment to hydrophilic surfaces and increased adhesion to a hydrophobic surface.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be83/183934/24ea16add85d/aem00064-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be83/183934/16b8b14d042a/aem00064-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be83/183934/699fb28eac22/aem00064-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be83/183934/24ea16add85d/aem00064-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be83/183934/16b8b14d042a/aem00064-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be83/183934/699fb28eac22/aem00064-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be83/183934/24ea16add85d/aem00064-0065-a.jpg

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