Azavache Venezuela, Cuevas Carmen, Bosch Virgilio
Sección de Lipidología, Instituto de Medicina Experimental. Universidad Central de Venezuela, Caracas, Venezuela.
Arch Latinoam Nutr. 2007 Mar;57(1):5-9.
Mammals along their early postnatal period develop a substantial amount of a very active brown adipose tissue (BAT). Through this work we explored the possibility that BAT may function as a long chain polyunsaturated fatty acids reservoir (LC-PUFA) during the rapid growth of brain structures. In new born rats 1, 6, 12 and 20 days old, we analyzed fatty acid percentage of triglycerides (TG) and phospholipid fractions, and the absolute amount of TG. In 6 day old rats we also evaluated the extend of further desaturation of 1-14C linoleic acid administered by intraperitoneal injection. Results demonstrated a drastic increase of TG concentration during experimental period (1,5; 40; 118; 120 mg/g wet weight) and LC-PUFA percentage was higher in "1 and 6" than "12 and 20" days old rats (16-17% vs 5%). Our results showed that BAT stored important amounts of LC-PUFA. On the other hand, 1-14C linoleic acid incorporation was higher in liver than BAT. In contrast, the desaturated products of 1-14C linoleic acid /1-14C linoleic acid ratio was greater in BAT than liver (>4). This could indicate that BAT synthesizes LC-PUFA in addition to store it. In summary we demonstrated than BAT is an important reservoir of LC-PUFA during postnatal brain growth.
哺乳动物在出生后的早期阶段会发育出大量非常活跃的棕色脂肪组织（BAT）。通过这项研究，我们探讨了在脑结构快速生长期间，棕色脂肪组织可能作为长链多不饱和脂肪酸（LC-PUFA）储存库的可能性。我们分析了1日龄、6日龄、12日龄和20日龄新生大鼠甘油三酯（TG）和磷脂组分中的脂肪酸百分比以及TG的绝对含量。对于6日龄的大鼠，我们还评估了通过腹腔注射给予的1-14C亚油酸进一步去饱和的程度。结果表明，在实验期间TG浓度急剧增加（1.5、40、118、120mg/g湿重），并且“1日龄和6日龄”大鼠的LC-PUFA百分比高于“12日龄和20日龄”大鼠（16 - 17%对5%）。我们的结果表明，棕色脂肪组织储存了大量的LC-PUFA。另一方面，肝脏中1-14C亚油酸的掺入量高于棕色脂肪组织。相反，棕色脂肪组织中1-14C亚油酸的去饱和产物/1-14C亚油酸的比例大于肝脏（>4）。这可能表明棕色脂肪组织除了储存LC-PUFA外还能合成LC-PUFA。总之，我们证明了在出生后脑生长期间，棕色脂肪组织是LC-PUFA的重要储存库。
