Peng L, Wang W, Bai L
Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
Int J Lab Hematol. 2007 Oct;29(5):361-8. doi: 10.1111/j.1751-553X.2007.00868.x.
The Z2 Coulter Counter (Z2) is a new, single-channel semi-automated electronic counter using the Coulter principle, providing both count and concentration results of cells, as well as providing size distribution of the cell population. The Z2 was evaluated according to guidelines published by the International Committee for Standardisation in Haematology. The results demonstrated excellent linearity for WBC and RBC (r > 0.999). The Z2 showed excellent precision, with a within-run, between-run and overall coefficient of variance less than 2%. Carryover was generally acceptable (<1%). WBC and RBC were stable after blood storage for 48 h at 4 degrees C and room temperature. There was almost no interference for WBC, RBC at high concentrations of bilirubin (178.5 mumol/l) or triglyceride (14.8 mmol/l) (P > 0.05). The bias of coincidence correction in WBC and RBC counting with the Z2 was less than 1%. Correlation between the Z2 and Sysmex F-820 or SE-9500 was excellent (r > 0.996) for WBC and RBC counting. Our results indicate that the Z2 has good features of precision, accuracy, linearity and good comparison with other haematology analysers, and could be used as a calibrating instrument for WBC and RBC counting.
Z2库尔特计数器（Z2）是一款新型单通道半自动电子计数器，采用库尔特原理，可提供细胞计数和浓度结果，以及细胞群体的大小分布。Z2依据国际血液学标准化委员会发布的指南进行评估。结果表明，白细胞（WBC）和红细胞（RBC）具有出色的线性关系（r>0.999）。Z2显示出卓越的精密度，批内、批间和总体变异系数均小于2%。携带污染通常可接受（<1%）。白细胞和红细胞在4℃和室温下储存48小时后保持稳定。在高浓度胆红素（178.5μmol/l）或甘油三酯（14.8mmol/l）情况下，对白细胞和红细胞几乎没有干扰（P>0.05）。使用Z2进行白细胞和红细胞计数时，重合校正偏差小于1%。在白细胞和红细胞计数方面，Z2与Sysmex F - 820或SE - 9500的相关性极佳（r>0.996）。我们的结果表明，Z2具有精密度、准确性、线性良好的特点，并且与其他血液学分析仪比较良好，可作为白细胞和红细胞计数的校准仪器。
