Wang Hui, Meng Qing H, Gordon John R, Khandwala Hasnain, Wu Lingyun
Department of Pharmacology, University of Saskatchewan, Saskatoon, 107 Wiggins Road, Canada S7N 5E5.
Clin Biochem. 2007 Nov;40(16-17):1232-9. doi: 10.1016/j.clinbiochem.2007.07.016. Epub 2007 Aug 11.
To determine the effect of methylglyoxal (MG) on cytokine production by, and apoptosis of, neutrophils from type 2 diabetes mellitus (T2DM) patients.
The levels of plasma MG, cytokines released by isolated neutrophils and the apoptotic status of neutrophils were determined.
The higher level of plasma MG in T2DM patients was correlated positively with glycated hemoglobin levels, fasting plasma glucose levels and urine albumin/creatinine ratios. The basal levels of cytokines released from neutrophils were markedly higher in patients. MG treatment of the neutrophils isolated from diabetic patients either did not alter, or decreased, the production of cytokines. In contrast, MG induced the release of cytokines from neutrophils of non-diabetics. Moreover, the neutrophils from T2DM patients showed a greater proclivity for apoptosis, which was further increased by in vitro MG treatment.
MG stimulated neutrophils to release more cytokines, which might play a role in the development of infection in T2DM.
确定甲基乙二醛（MG）对2型糖尿病（T2DM）患者中性粒细胞产生细胞因子及凋亡的影响。
测定血浆MG水平、分离的中性粒细胞释放的细胞因子以及中性粒细胞的凋亡状态。
T2DM患者血浆MG水平较高，与糖化血红蛋白水平、空腹血糖水平及尿白蛋白/肌酐比值呈正相关。患者中性粒细胞释放的细胞因子基础水平明显更高。用MG处理糖尿病患者分离出的中性粒细胞，要么不会改变细胞因子的产生，要么会使其减少。相比之下，MG可诱导非糖尿病患者中性粒细胞释放细胞因子。此外，T2DM患者的中性粒细胞表现出更大的凋亡倾向，体外MG处理可使其进一步增加。
MG刺激中性粒细胞释放更多细胞因子，这可能在T2DM感染的发生发展中起作用。
