Department of Cadre's Ward, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, P. R. China.
Institute of Nanomedicine and Nanobiosensing; MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, P. R. China.
ChemistryOpen. 2022 May;11(5):e202200055. doi: 10.1002/open.202200055.
Accurate monitoring of methylglyoxal (MGO) at cell and living level was crucial to reveal its role in the pathogenesis of diabetes since MGO was closely related to diabetes. Herein, a ratiometric fluorescence strategy was constructed based on the capture probe 2,3-diaminonaphthalene (DAN) for the specific detection of MGO. Compared to the fluorescent probes with a single emission wavelength, the ratiometric mode by monitoring two emissions can effectively avoid the interference from the biological background, and provided additional self-calibration ability, which can realize accurate detection of MGO. The proposed method showed a good linear relationship in the range of 0-75 μm for MGO detection, and the limit of detection was 0.33 μm. DAN responded to MGO with good specificity and was successfully applied for detecting the ex vivo MGO level in plasma of KK-Ay mice as a type II diabetes model. Besides, the prepared DAN test strip can be visualized for rapid semi-quantitative analysis of MGO using the naked eye. Furthermore, human skin fibroblasts and HeLa cells were utilized for exogenous MGO imaging, and ex vivo MGO imaging was performed on tissues of KK-Ay mice. All results indicated that the DAN-based ratiometric fluorescence probe can be used as a potential method to detect the level of MGO, thus enabling indications for the occurrence of diabetes and its complications.
准确监测甲基乙二醛 (MGO) 在细胞和活体水平上的含量对于揭示其在糖尿病发病机制中的作用至关重要，因为 MGO 与糖尿病密切相关。在此，基于捕获探针 2,3-二氨基萘 (DAN) 构建了一种用于特异性检测 MGO 的比率荧光策略。与具有单一发射波长的荧光探针相比，通过监测两个发射的比率模式可以有效地避免生物背景的干扰，并提供额外的自校准能力，从而可以实现 MGO 的准确检测。所提出的方法在用于检测 MGO 的 0-75 μm 范围内显示出良好的线性关系，检测限为 0.33 μm。DAN 对 MGO 的响应具有良好的特异性，并成功应用于检测 II 型糖尿病模型 KK-Ay 小鼠血浆中的体外 MGO 水平。此外，制备的 DAN 测试条可用于使用肉眼进行 MGO 的快速半定量分析。此外，用人皮肤成纤维细胞和 HeLa 细胞进行外源性 MGO 成像，并对 KK-Ay 小鼠组织进行了体外 MGO 成像。所有结果表明，基于 DAN 的比率荧光探针可用于检测 MGO 的水平，从而为糖尿病及其并发症的发生提供指示。
