Li Guangyong, Xi Ning, Wang Donna H
Department of Electrical and Computer Engineering, University of Pittsburgh, 348 Benedum Hall, Pittsburgh, PA 15261, USA.
Med Clin North Am. 2007 Sep;91(5):929-36. doi: 10.1016/j.mcna.2007.05.003.
The use of a functionalized tip to measure the force of the interaction between ligands and receptors by atomic force microscopy has been discussed for more than a decade, and single-molecule recognition using a functionalized tip from processed samples is achievable now. Techniques for detecting and characterizing specific individual molecules from a living cell are still developing and are discussed in this article. Because many diseases have their roots at the molecular scale and are best understood as a malfunctioning of biologic nanomachines, these techniques should find widespread use in basic biomedical research once the remaining barriers are overcome.
利用功能化探针通过原子力显微镜测量配体与受体之间相互作用的力这一方法已被讨论了十多年，现在使用经过处理的样品中的功能化探针实现单分子识别是可行的。从活细胞中检测和表征特定单个分子的技术仍在不断发展，本文将对此进行讨论。由于许多疾病起源于分子尺度，并且最好理解为生物纳米机器的故障，一旦克服了剩下的障碍，这些技术应该会在基础生物医学研究中得到广泛应用。
