Ludwig M, Dettmann W, Gaub H E
Lehrstuhl für Angewandte Physik, Ludwig Maximilians Universität, München, Germany.
Biophys J. 1997 Jan;72(1):445-8. doi: 10.1016/S0006-3495(97)78685-5.
The contrast in atomic force microscope images arises from forces between the tip and the sample. It was shown recently that specific molecular interaction forces may be measured with the atomic force microscope; consequently, we use such forces to map the distribution of binding partners on samples. Here we demonstrate this concept by imaging a streptavidin pattern with a biotinylated tip in a novel imaging mode called affinity imaging. In this mode topography, adhesion, and sample elasticity are extracted online from local force scans. We show that this technique allows the separation of these values and that the measured binding pattern is based on specific molecular interactions.
原子力显微镜图像中的对比度源于探针与样品之间的力。最近的研究表明，特定的分子相互作用力可以通过原子力显微镜进行测量；因此，我们利用这些力来绘制样品上结合配体的分布图。在这里，我们通过在一种称为亲和成像的新型成像模式下，用生物素化的探针成像链霉亲和素图案来证明这一概念。在这种模式下，形貌、粘附力和样品弹性可从局部力扫描中在线提取。我们表明，该技术能够分离这些值，并且所测量的结合图案基于特定的分子相互作用。
