Nishizawa Koji, Torii Kosuke, Kawasaki Aya, Katada Masanori, Ito Minoru, Terashita Kenzo, Aiso Sadakazu, Matsuoka Masaaki
Noevir-Keio Research Laboratory, Noevir Co., Ltd, Shinjuku-ku, Tokyo 160-8582, Japan.
Biol Pharm Bull. 2007 Sep;30(9):1758-62. doi: 10.1248/bpb.30.1758.
Cordyceps sinensis (CS) has been known as a component of traditional medicines that elicit various biological effects such as anti-fatigue, immunomodulatory, and hypoglycemic actions. Since it has been well-established that fatigue is closely related to depression, we used the tail suspension test (TST) in mice to examine the antidepressant-like effects of hot water extract (HWCS) and supercritical fluid extract (SCCS) of CS. Immobility time in the TST was reduced by administration of SCCS (2.5-10 ml/kg, p.o.) dose-dependently though it was not reduced by treatment with HWCS (500-2000 mg/kg, p.o.). Neither HWCS nor SCCS altered locomotor activity in the open field test, excluding the possibility that the effect of SCCS is due to activation of locomotion. Pretreatment with prazosin (an adrenoreceptor antagonist) or sulpiride (a dopamine D2 receptor antagonist) reduced the effect of SCCS on the immobility time. In contrast, pretreatment with p-chlorophenylalanine (p-CPA, a serotonin synthesis inhibitor) did not alter the anti-immobility effect of SCCS. The last finding is consistent with an additional observation that SCCS had no effect on head twitch response induced by 5-hydroxy-L-tryptophan in mice. Taken altogether, these results suggest that SCCS may elicit an antidepressant-like effect by affecting the adrenergic and dopaminergic systems, but not by affecting the serotonergic system.
冬虫夏草（CS）一直被认为是传统药物的一种成分，具有多种生物学效应，如抗疲劳、免疫调节和降血糖作用。由于已经充分证实疲劳与抑郁症密切相关，我们使用小鼠悬尾实验（TST）来研究冬虫夏草热水提取物（HWCS）和超临界流体提取物（SCCS）的抗抑郁样作用。口服给予SCCS（2.5 - 10 ml/kg）可剂量依赖性地减少TST中的不动时间，而口服给予HWCS（500 - 2000 mg/kg）则无此作用。HWCS和SCCS在旷场实验中均未改变运动活性，排除了SCCS的作用是由于运动激活的可能性。用哌唑嗪（一种肾上腺素能受体拮抗剂）或舒必利（一种多巴胺D2受体拮抗剂）预处理可降低SCCS对不动时间的影响。相反，用对氯苯丙氨酸（p - CPA，一种血清素合成抑制剂）预处理并未改变SCCS的抗不动作用。最后这一发现与另一观察结果一致，即SCCS对小鼠中5 - 羟基 - L - 色氨酸诱导的头部抽搐反应无影响。综上所述，这些结果表明SCCS可能通过影响肾上腺素能和多巴胺能系统而非血清素能系统产生抗抑郁样作用。
