Keller Philipp J, Pampaloni Francesco, Stelzer Ernst H K
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Nat Methods. 2007 Oct;4(10):843-6. doi: 10.1038/nmeth1087. Epub 2007 Sep 9.
We present an experimental investigation of microtubule dynamic instability in three dimensions, based on laser light-sheet fluorescence microscopy. We introduce three-dimensional (3D) preparation of Xenopus laevis egg extracts in Teflon-based cylinders and provide algorithms for 3D image processing. Our approach gives experimental access to the intrinsic dynamic properties of microtubules and to microtubule population statistics in single asters. We obtain evidence for a stochastic nature of microtubule pausing.
我们基于激光片层荧光显微镜对三维空间中微管的动态不稳定性进行了实验研究。我们介绍了在基于聚四氟乙烯的圆柱体中对非洲爪蟾卵提取物进行三维（3D）制备，并提供了三维图像处理算法。我们的方法使实验能够获取微管的内在动态特性以及单个星状体中微管群体的统计信息。我们获得了微管暂停具有随机性的证据。
