Keller Philipp J, Pampaloni Francesco, Stelzer Ernst Hk
EMBL Heidelberg, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Curr Opin Cell Biol. 2006 Feb;18(1):117-24. doi: 10.1016/j.ceb.2005.12.012. Epub 2006 Jan 4.
Novel technologies are required for three-dimensional cell biology and biophysics. By three-dimensional we refer to experimental conditions that essentially try to avoid hard and flat surfaces and favour unconstrained sample dynamics. We believe that light-sheet-based microscopes are particularly well suited to studies of sensitive three-dimensional biological systems. The application of such instruments can be illustrated with examples from the biophysics of microtubule dynamics and three-dimensional cell cultures. Our experience leads us to suggest that three-dimensional approaches reveal new aspects of a system and enable experiments to be performed in a more physiological and hence clinically more relevant context.
三维细胞生物学和生物物理学需要新技术。我们所说的三维是指那些基本上试图避免坚硬和平坦表面并有利于无约束样本动态变化的实验条件。我们认为基于光片的显微镜特别适合用于研究敏感的三维生物系统。此类仪器的应用可以通过微管动力学生物物理学和三维细胞培养的例子来说明。我们的经验使我们认为，三维方法能揭示系统的新方面，并能在更符合生理、因而在临床上更相关的背景下进行实验。
