Gallice P M, Monti J P, Braguer D L, Baz M, Elsen R E, Berland Y F, Crevat A D
Biophysics Laboratory, UFR de Pharmacie, Marseille, France.
Int J Artif Organs. 1991 Dec;14(12):754-8.
The middle-molecular-weight uremic toxins which accumulate in uremic plasma seem to be associated with various uremic disorders such as uremic neuropathy and defects in the sodium pump. By a multi-step chromatographic method, two fractions of these toxins were isolated and studied because one inhibits microtubule formation in vitro (fraction 2-5), and the other impairs the sodium pump in living erythrocytes (fraction 2-3). An additional chromatographic method allows the separation of these fractions and isolation of two components: fractions 2-3-V and 2-5-III. Analyses by UV and 1H NMR spectrometry identified these compounds as two different ascorbic acid derivatives. 2-3-V is not yet totally identified and 2-5-III corresponds to ascorbic acid 2-sulfate. These two metabolites exert no toxic effects but they have the same chromatographic behavior as uremic toxins.
积聚在尿毒症血浆中的中分子尿毒症毒素似乎与各种尿毒症病症相关，如尿毒症神经病变和钠泵缺陷。通过多步色谱法，分离并研究了这些毒素的两个组分，因为其中一个在体外抑制微管形成（组分2 - 5），另一个损害活红细胞中的钠泵（组分2 - 3）。另一种色谱方法可分离这些组分并分离出两种成分：组分2 - 3 - V和2 - 5 - III。通过紫外光谱和1H核磁共振光谱分析确定这些化合物为两种不同的抗坏血酸衍生物。2 - 3 - V尚未完全鉴定出来，2 - 5 - III对应于抗坏血酸2 - 硫酸盐。这两种代谢物没有毒性作用，但它们具有与尿毒症毒素相同的色谱行为。
