Gallice P M, Monti J P, Braguer D L, Monti H M, Elsen R E, Berland Y F, Crevat A D
Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
Clin Chem. 1990 Jul;36(7):1369-72.
Among uremic toxins in the middle molecular mass range, 1H, 13C-nuclear magnetic resonance, ultraviolet spectrometry, and chromatographic analyses allow identification of the main component of the so-called "2-5-3 fraction" as ascorbic acid 2-sulfate, a conjugated metabolite of ascorbic acid. We previously (Clin Nephrol 1986;25:212-8) showed an inhibitory effect of the 2-5-3 fraction on microtubule formation. Therefore, we tested the action of ascorbic acid 2-sulfate and its synthetized enantiomers on tubulin polymerization. Because these molecules did not exert any inhibitory effect, we hypothesize that the 2-5-3 fraction is a mixture of compounds in which only a very low quantity of the inhibitory factor is present.
在中等分子量范围内的尿毒症毒素中，氢-1、碳-13核磁共振、紫外光谱分析和色谱分析能够鉴定出所谓“2-5-3组分”的主要成分是抗坏血酸2-硫酸盐，它是抗坏血酸的一种共轭代谢产物。我们之前（《临床肾脏病学》1986年；25:212-218）报道过2-5-3组分对微管形成具有抑制作用。因此，我们测试了抗坏血酸2-硫酸盐及其合成对映体对微管蛋白聚合的作用。由于这些分子未发挥任何抑制作用，我们推测2-5-3组分是一种化合物混合物，其中仅存在极少量的抑制因子。
