Kato M, Pu M Y, Isobe K, Iwamoto T, Nagase F, Lwin T, Zhang Y H, Hattori T, Yanagita N, Nakashima I
Department of Immunology, Nagoya University School of Medicine, Japan.
Cell Immunol. 1994 Nov;159(1):15-25. doi: 10.1006/cimm.1994.1291.
The immunoregulatory action of saikosaponin-d (SSd), which was isolated from the root of Bupleurum falcatum L. and has a steroid-like structure, was examined on splenic T lymphocytes of C57BL/6 mice. SSd displayed a definite action in vitro to bidirectionally control the growth response of T lymphocytes stimulated by concanavalin A, anti-CD3 monoclonal antibody, and calcium ionophore A23187 plus phorbol 12-myristate 13-acetate. Low concentrations (1-3 micrograms/ml) of SSd upregulated the responses to suboptimum stimuli of agonists, particularly during the relatively late stage of the responses, whereas it downregulated the responses to supraoptimal stimuli. Under appropriate experimental conditions, SSd promoted interleukin-2 (IL-2) production and IL-2 receptor expression. It also accelerated c-fos gene transcription, but it did not modulate the level of tyrosine phosphorylation of cellular proteins. We concluded from these results that SSd uniquely modulates T lymphocyte function and that at least one target of the action of SSd is located at or before the step of c-fos gene transcription and after T-cell receptor/CD3-mediated protein tyrosine kinase activation.
从柴胡根部分离得到的具有类固醇样结构的柴胡皂苷d（SSd），对C57BL/6小鼠脾T淋巴细胞的免疫调节作用进行了研究。SSd在体外对由刀豆球蛋白A、抗CD3单克隆抗体以及钙离子载体A23187加佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯刺激的T淋巴细胞生长反应具有明确的双向调控作用。低浓度（1 - 3微克/毫升）的SSd上调了对激动剂次优刺激的反应，尤其是在反应的相对后期，而它下调了对超优刺激的反应。在适当的实验条件下，SSd促进白细胞介素-2（IL - 2）的产生和IL - 2受体的表达。它还加速了c - fos基因的转录，但未调节细胞蛋白酪氨酸磷酸化水平。从这些结果我们得出结论，SSd独特地调节T淋巴细胞功能，并且SSd作用的至少一个靶点位于c - fos基因转录步骤或之前以及T细胞受体/CD3介导的蛋白酪氨酸激酶激活之后。
