Kato M, Pu M Y, Isobe K, Hattori T, Yanagita N, Nakashima I
Department of Immunology, Nagoya University School of Medicine, Japan.
Immunopharmacology. 1995 Apr;29(3):207-13. doi: 10.1016/0162-3109(95)00059-3.
We examined the immunoregulatory action of saikosaponin-d (SSd), which was isolated from the root of Bupleurum talcatum L. and had a steroid-like structure, on murine thymocytes, and compared the action with that on spleen cells. Constitutive DNA synthesis or the growth response stimulated with anti-CD3mAb of thymocytes were down-regulated by 3 micrograms/ml SSd, whereas with spleen cells these were up-regulated by the same concentration of SSd. On the other hand, 3 micrograms/ml of SSd greatly up-regulated the growth response and interleukin 2 (IL-2)/interleukin 4 (IL-4) production induced through a receptor-bypassed pathway by calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) in thymocytes, whereas it only slightly up-regulated them in spleen cells. Moreover, the same concentration of SSd inhibited DNA fragmentation in thymocytes induced by A23187 or PMA. These results suggest a unique cell type-dependent immuno-modulatory action of SSd.
我们研究了从柴胡根部分离得到的、具有类固醇样结构的柴胡皂苷 -d（SSd）对小鼠胸腺细胞的免疫调节作用，并将其作用与对脾细胞的作用进行了比较。胸腺细胞的组成性DNA合成或抗CD3单克隆抗体刺激的生长反应被3微克/毫升的SSd下调，而相同浓度的SSd对脾细胞的这些反应则起到上调作用。另一方面，3微克/毫升的SSd极大地上调了钙离子载体A23187加佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）通过受体旁路途径诱导的胸腺细胞生长反应以及白细胞介素2（IL - 2）/白细胞介素4（IL - 4）的产生，而在脾细胞中它只是轻微上调。此外，相同浓度的SSd抑制了由A23187或PMA诱导的胸腺细胞中的DNA片段化。这些结果表明SSd具有独特的细胞类型依赖性免疫调节作用。
