van Papendorp C L, Cameron I T, Davenport A P, King A, Barker P J, Huskisson N S, Gilmour R S, Brown M J, Smith S K
Department of Obstetrics and Gynaecology, University of Cambridge Clinical School, Rosie Maternity Hospital.
J Endocrinol. 1991 Dec;131(3):507-11. doi: 10.1677/joe.0.1310507.
The aim of this study was to investigate the localization of endothelin-like immunoreactivity (ET-IR) in human placenta, chorion and amnion and to compare the endogenous concentration of immunoreactive endothelin (ET) in these tissues before and after the onset of labour. ET-IR was detected in the endothelium of stem vessels in placental villi, as well as in decidual stromal cells in the basal maternal plate, by immunocytochemistry using primary polyclonal rabbit antibody. A specific radioimmunoassay was used to detect endogenous concentration of ET in homogenized placental tissues. The endogenous concentration of ET-IR was significantly greater in amnion than in chorion and placenta (amnion 249 +/- 13 fmol/g; chorion 190 +/- 11 fmol/g; placenta 169 +/- 14 fmol/g; means +/- S.E.M.; n = 12; P less than 0.01). No significant difference was seen before or after the onset of labour. The detection of ET-IR in placenta, chorion and amnion suggests that the ETs may play a role in the paracrine control of human uterine function.
本研究的目的是调查内皮素样免疫反应性（ET-IR）在人胎盘、绒毛膜和羊膜中的定位，并比较分娩前后这些组织中免疫反应性内皮素（ET）的内源性浓度。使用兔多克隆一抗通过免疫细胞化学方法在胎盘绒毛干血管的内皮以及基底蜕膜板的蜕膜基质细胞中检测到了ET-IR。采用特异性放射免疫分析法检测匀浆胎盘组织中ET的内源性浓度。羊膜中ET-IR的内源性浓度显著高于绒毛膜和胎盘（羊膜249±13 fmol/g；绒毛膜190±11 fmol/g；胎盘169±14 fmol/g；均值±标准误；n = 12；P<0.01）。分娩前后未见显著差异。在胎盘、绒毛膜和羊膜中检测到ET-IR表明ET可能在人子宫功能的旁分泌调节中发挥作用。
