Harman K, Katnick J, de la Torre J C
Physiotherapy Program, University of Ottawa, Ont., Canada.
J Neurosci Methods. 1991 Jul;38(2-3):107-10. doi: 10.1016/0165-0270(91)90160-2.
A quick and accurate computer-assisted method of quantifying the number of myelinated axons in normal and experimental or regenerated peripheral nerve cross-sections is described. Using an IBM-PC, quantitation software and a light microscope with a camera lucida attachment, the number of axons in a sciatic nerve can be calculated in fifteen minutes. Nine nerve samples with various nerve diameters and axon densities were used to test the technique. Total counts (actual count) were compared to the number of axons estimated by the line-sampling technique (projected count) and the two groups varied up to 15%. The principle advantage of this method is that it saves time by eliminating photography and performing total counts. The technique can be applied to normal and regenerated peripheral nerve.
本文描述了一种快速准确的计算机辅助方法，用于量化正常、实验性或再生性周围神经横切面中有髓轴突的数量。使用一台IBM-PC、定量软件和一台配有明视野描绘器的光学显微镜，在15分钟内就能计算出坐骨神经中的轴突数量。使用了9个具有不同神经直径和轴突密度的神经样本对该技术进行测试。将总计数（实际计数）与通过线抽样技术估计的轴突数量（预计计数）进行比较，两组数据的差异高达15%。该方法的主要优点是通过省去摄影步骤并进行总计数来节省时间。该技术可应用于正常和再生的周围神经。
