Venkateswaran K, Nakano H, Kiiyukia C, Kawakami H, Hashimoto H
Department of Food Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, Japan.
Microbios. 1991;68(275):73-85.
The role of sinking particles in the distribution of motile Aeromonas species was studied during the winter season. Various environmental parameters and microbial populations were investigated to elucidate the relationship with motile aeromonads. Motile Aeromonas species were enumerated by most probable number technique with alkaline peptone water as the enrichment medium and modified pril-xylose ampicillin agar as the plating medium. Aeromonas species were isolated in a water column in any one of the two procedures but sediment and plankton samples exhibited an irregular isolation pattern for these organisms. Aeoromonas species were continuously isolated in sinking particles with the highest counts during January. Of the 206 isolates identified, three known motile Aeromonas species were observed of which A. caviae accounted for 51.4% of the total. Toxin characterization showed that 20% of the strains produced haemolysin as well as cytotoxin, and A. hydrophila was highly toxigenic. Statistical analyses revealed that nutrients govern the distribution of Aeromonas. It may be that riverine discharge influences the distribution of motile aeromonads in this environment.
在冬季研究了下沉颗粒在运动性气单胞菌属分布中的作用。研究了各种环境参数和微生物种群，以阐明与运动性气单胞菌的关系。采用最可能数技术，以碱性蛋白胨水为富集培养基，改良普里木糖氨苄青霉素琼脂为平板培养基，对运动性气单胞菌属进行计数。气单胞菌属在水柱中可通过两种方法中的任何一种分离得到，但沉积物和浮游生物样本中这些微生物的分离模式不规则。气单胞菌属在下沉颗粒中持续分离得到，1月份数量最多。在鉴定出的206株分离物中，观察到三种已知的运动性气单胞菌属，其中豚鼠气单胞菌占总数的51.4%。毒素特性表明，20%的菌株产生溶血素和细胞毒素，嗜水气单胞菌具有高度产毒性。统计分析表明，营养物质控制着气单胞菌的分布。可能是河流排放影响了该环境中运动性气单胞菌的分布。
