Tao R V, Holleschau A M, Rathbun W B
Department of Ophthalmology, University of Illinois, Chicago.
Ophthalmic Res. 1991;23(5):272-83. doi: 10.1159/000267123.
This investigation compared the effects of two types of aldose reductase inhibitors on several biochemical parameters in naphthalene-induced cataract of the rat over a time span of 102 days of treatment. Feeding of naphthalene daily to brown Norway rats resulted in gradual, progressive development of zonular opacities. As compared to control animals, the values of soluble protein, soluble glutathione (total of oxidized plus reduced) and activities of glutathione peroxidase and glutathione reductase were decreased in rats fed either naphthalene or naphthalene + FK366, a carboxylic-acid-type aldose reductase inhibitor. In marked contrast, treatment with A11576, a hydantoin-type aldose reductase inhibitor, maintained the values of most parameters (with one exception) at levels that were similar to those of the controls, and all lenses remained clear. A decline of glutathione was noted in all naphthalene-fed rats, irrespective of whether these animals had been treated with an aldose reductase inhibitor. The great decrease of glutathione with A11576 suggests that this inhibitor acts at some step in naphthalene metabolism following formation of naphthalene epoxide.
本研究比较了两种醛糖还原酶抑制剂在102天的治疗时间内，对萘诱导的大鼠白内障的几种生化参数的影响。每天给棕色挪威大鼠喂食萘会导致晶状体小带混浊逐渐发展。与对照动物相比，喂食萘或萘+FK366（一种羧酸型醛糖还原酶抑制剂）的大鼠的可溶性蛋白、可溶性谷胱甘肽（氧化型加还原型的总和）以及谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性值均降低。与之形成显著对比的是，用乙内酰脲型醛糖还原酶抑制剂A11576治疗后，大多数参数（有一个例外）的值维持在与对照组相似的水平，并且所有晶状体均保持透明。在所有喂食萘的大鼠中均观察到谷胱甘肽减少，无论这些动物是否接受了醛糖还原酶抑制剂治疗。使用A11576后谷胱甘肽大幅减少，这表明该抑制剂在萘环氧化物形成后的萘代谢的某个步骤起作用。
